Journal: bioRxiv

Article Title: Pyramidal cell types and 5-HT 2A receptors are essential for psilocybin’s lasting drug action

doi: 10.1101/2024.11.02.621692

Figure Lengend Snippet: a, Single-cell transcript counts summed from the mouse ACA, ALM, ORB, and PL-ILA regions, extracted from the SMART-Seq data from Allen Institute. b, Viral injection strategy for inducing 5-HT 2A receptor knockout or control in GFP+ cells in medial frontal cortex. Inset, post hoc histology. c, Htr2a mRNA levels in GFP+ cells quantified using qPCR following FACS. Circle, individual animal. d, Whole-cell voltage-clamp recordings of spontaneous EPSCs from GFP+ layer 5 pyramidal neurons in baseline, after bath application of 20 µM 5-HT, or after bath application of 20 µM 5-HT and 100 nM MDL100,907. Circle, individual cell. e, Head-twitch response measured in control animals after saline (gray) or psilocybin (red) and in animals with bilateral medial frontal cortex-specific 5-HT 2A receptor knockout after saline (light gray) or psilocybin (blue). Circle, individual animal. Mean and s.e.m. f, Similar to e for learned helplessness. g, Similar to e for tail suspension. *, p < 0.05. **, p < 0.01. ***, p < 0.001. Sample size n values are provided in Methods. Statistical analyses are provided in Supplementary Table 1.

Article Snippet: The free-floating sections were washed 3 times with 0.3% TX-100/PBS prior to a 1 hour incubation in blocking buffer (5% normal donkey serum in 0.3% TX-100/PBS) and then incubated overnight at room temperature with anti-rabbit HTR2A antibody (1:250 dilution, #RA24288, Neuromics).

Techniques: Injection, Knock-Out, Control, Saline, Suspension

Journal: Ecotoxicology and environmental safety

Article Title: Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats.

doi: 10.1016/j.ecoenv.2021.112806

Figure Lengend Snippet: Fig. 4. Developmental exposure to low-dose ([C8MIM] [PF6]) increased the expression of NMDA receptors. (A) The mRNA level of major receptors (nmdar1, nmdar2a, nmdar2b for NMDA receptor; drd1 and drd2 for dopamine receptor; 5-ht2ar for 5-HT receptor; gabaarα1 for GABA receptor) for main neurotransmitters in hippocampus and prefrontal cortex. (B) The protein levels of NMDA receptors (NMDAR1, NMDAR2A, and NMDAR2B) in the hippocam pus and prefrontal cortex. (C) The protein levels of dopa mine receptors (DRD1 and DRD2), 5-HT receptors (5- HT2AR), and GABA receptors (GABAARα1) in the hippo campus and prefrontal cortex. (D) Statistical analysis of protein expression. n = 3 for each group. Student’s t-test was performed for the analysis. The data are presented as the mean ± SEM. *p＜0.05, **p＜0.01, ***p＜0.001.

Article Snippet: Membranes were treated with 5% skim milk for 1 h, followed by incubation with primary antibodies against NMDAR1 (ab109182, Abcam), NMDAR2A (ab124913, Abcam), NMDAR2B (ab65783, Abcam), DRD1 (ab81296, Abcam), DRD2 (ab30743, Abcam), 5-HT2AR (PB9599, Boster), GABAARα1 (ab252430, Abcam), PSD95 (36233S, Cell Signalling Technology), IBA1 (GTX100042, GeneTex), GFAP (BA0056, Boster), NeuN (ab177487, Abcam), MBP (BA0094, Boster), IFN-γ (PAA033Ra01, Cloud-clone Corp.), IL-1β (PAA563Ra01, Cloud-clone Corp.), TNF-α (PAA133Ra01, cloud-clone corp.), NF-ĸB (66535-1-lg, Proteintech), Histone H3 (AF0009, Beyotime), NRF2 (bs-1074R-1, bioss), PPARγ(ab272718, Abcam), COX2 (ab179800, Abcam), iNOS (ab178945, Abcam), Caspase 3 (9662s, CST), Bax (SC-7480, SANTA CRUZ), Bcl-2 (sc7382, SANTA CRUZ), GAPDH (5174T, CST), and β-actin (3700s, CST) at a 1:1000 dilution overnight at 4 ◦C.

Techniques: Expressing